Search results for " bioreactor"
showing 10 items of 149 documents
Improvement of osteogenic differentiation of human mesenchymal stem cells on composite poly l-lactic acid/nano-hydroxyapatite scaffolds for bone defe…
2020
Tissue engineering offers new approaches to repair bone defects, which cannot be repaired physiologically, developing scaffolds that mimic bone tissue architecture. Furthermore, biomechanical stimulation induced by bioreactor, provides biomechanical cues that regulate a wide range of cellular events especially required for cellular differentiation and function. The improvement of human mesenchymal stem cells (hMSCs) colonization in poly-L-lactic-acid (PLLA)/nano- hydroxyapatite (nHA) composite scaffold was evaluated in terms of cell proliferation (dsDNA content), bone differen- tiation (gene expression and protein synthesis) and ultrastructural analysis by comparing static (s3D) and dynamic…
In Vitro Regeneration of Capparis spinosa L. by Using a Temporary Immersion System
2019
Three caper (Capparis spinosa L.) biotypes grown on the Sicilian island of Salina (38°
Aeration control in membrane bioreactor for sustainable environmental footprint
2020
In this study different scenarios were scrutinized to minimize the energy consumption of a membrane bioreactor system for wastewater treatment. Open-loop and closed-loop scenarios were investigated by two-step cascade control strategies based on dissolved oxygen, ammonia and nitrite concentrations. An integrated MBR model which includes also the greenhouse gas formation/emission processes was applied. A substantial energy consumption reduction was obtained for the closed-loop scenarios (32% for Scenario 1 and 82% for Scenario 2). The air flow control based on both ammonia and nitrite concentrations within the aerobic reactor (Scenario 2) provided excellent results in terms of reduction of o…
Anaerobic membrane bioreactors (AnMBR) treating urban wastewater in mild climates
2020
[EN] Feasibility of an AnMBR demonstration plant treating urban wastewater (UWW) at temperatures around 25-30 degrees C was assessed during a 350-day experimental period. The plant was fed with the effluent from the pretreatment of a full-scale municipal WWTP, characterized by high COD and sulfate concentrations. Biodegradability of the UWW reached values up to 87%, although a portion of the biodegradable COD was consumed by sulfate reducing organisms. Effluent COD remained below effluent discharge limits, achieving COD removals above 90%. System operation resulted in a reduction of sludge production of 36-58% compared to theoretical aerobic sludge productions. The membranes were operated a…
A plant-wide modelling comparison between membrane bioreactors and conventional activated sludge
2020
Abstract A comprehensive plant-wide mathematical modelling comparison between conventional activated sludge (CAS) and Membrane bioreactor (MBR) systems is presented. The main aim of this study is to highlight the key features of CAS and MBR in order to provide a guide for an effective plant operation. A scenario analysis was performed to investigate the influence on direct and indirect greenhouse gas (GHG) emissions and operating costs of (i) the composition of inflow wastewater (scenario 1), (ii) operating conditions (scenario 2) and (iii) oxygen transfer efficiency (scenario 3). Scenarios show higher indirect GHG emissions for MBR than CAS, which result is related to the higher energy con…
Assessment and characterization of the bacterial community structure in advanced activated sludge systems
2019
Abstract The present study is aimed to assess and characterize the structure of bacterial community in advanced activated sludge systems. In particular, activated sludge samples were collected from an Integrated Fixed-film Activated Sludge – Membrane Bioreactor pilot plant under a University of Cape Town configuration with in-series anaerobic (Noair)/anoxic (Anox)/aerobic (Oxy) reactors – and further analyzed. The achieved results – based on Next Generation Sequencing (NGS) of 16S rDNA amplicons – revealed that the bacterial biofilm (bf) communities on plastic carriers of Oxy and Anox reactors had a greater diversity compared to suspended (sp) bacterial flocs of Oxy, Anox and Noair. Indeed,…
Applicability of a single‐use bioreactor compared to a glass bioreactor for the fermentation of filamentous fungi and evaluation of the reproducibili…
2021
Abstract The implementation of single‐use technologies offers several major advantages, e.g. prevention of cross‐contamination, especially when spore‐forming microorganisms are present. This study investigated the application of a single‐use bioreactor in batch fermentation of filamentous fungus Penicillium sp. (IBWF 040‐09) from the Institute of Biotechnology and Drug Research (IBWF), which is capable of intracellular production of a protease inhibitor against parasitic proteases as a secondary metabolite. Several modifications to the SU bioreactor were suggested in this study to allow the fermentation in which the fungus forms pellets. Simultaneously, fermentations in conventional glass b…
Integrated Fixed Film Activated Sludge (IFAS) membrane BioReactor: The influence of the operational parameters
2020
Abstract The present paper investigated an Integrated Fixed Film Activated Sludge (IFAS) Membrane BioReactor (MBR) system monitored for 340 days. In particular, the short-term effects of some operational parameters variation was evaluated. Results showed a decrease of the removal rates under low C/N values. Respirometry results highlighted that activated sludge was more active in the organic carbon removal. Conversely, biofilm has a key role during nitrification. The major fouling mechanism was represented by the cake deposition (irreversible).
Integrated membrane bioreactors modelling: A review on new comprehensive modelling framework
2021
International audience; Integrated Membrane Bioreactor (MBR) models, combination of biological and physical models, have been representing powerful tools for the accomplishment of high environmental sustainability. This paper, produced by the International Water Association (IWA) Task Group on Membrane Modelling and Control, reviews the state-of-the-art, identifying gaps for future researches, and proposes a new integrated MBR modelling framework. In particular, the framework aims to guide researchers and managers in pursuing good performances of MBRs in terms of effluent quality, operating costs (such as membrane fouling, energy consumption due to aeration) and mitigation of greenhouse gas…
Acclimatised rumen culture for raw microalgae conversion into biogas: Linking microbial community structure and operational parameters in anaerobic m…
2019
[EN] Ruminal fluid was inoculated in an Anaerobic Membrane Reactor (AnMBR) to produce biogas from raw Scenedesmus. This work explores the microbial ecology of the system during stable operation at different solids retention times (SRT). The 16S rRNA amplicon analysis revealed that the acclimatised community was mainly composed of Anaerolineaceae, Spirochaetaceae, Lentimicrobiaceae and Cloacimonetes fermentative and hydrolytic members. During the highest biodegradability achieved in the AnMBR (62%) the dominant microorganisms were Fervidobacterium and Methanosaeta. Different microbial community clusters were observed at different SRT conditions. Interestingly, syntrophic bacteria Gelria and …